Evaluation of MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker for characterizing Microsatellite Instability in Persian Lynch Syndrome patients

Evaluation of MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker for characterizing Microsatellite Instability in Persian Lynch Syndrome patients

نوع فایل:PDFتعداد صفحات:9
سال انتشار:1395
چکیده
Background Colorectal malignancies with high microsatellite instability (MSI-H), either hereditary (Lynchsyndrome) or sporadic, demonstrate betterprognosis, altered response to 5FU chemotherapy and altered operativeapproach. It is now recommended to perform MSI testing for all new cases of colorectal cancer regardless of beingcategorized as hereditary or sporadic. For MSI detection, immunohistochemistry or PCR-based protocols using acohort of various sets of STR markers are recommended. Here we aimed to evaluate a simplified protocol using justa single STR marker, MT1XT20 mononucleotide repeat, for detection of MSI in Lynch syndrome patients. Promegafive- marker MSI testing panel and Immunohistochemistry…