Investigation of tropane alkaloid production and expression of pmt gene, in Atropa belladonna cell suspension culture treated with Piriformospora indi

Investigation of tropane alkaloid production and expression of pmt gene, in Atropa belladonna cell suspension culture treated with Piriformospora indi

نوع فایل:PDFتعداد صفحات :9
سال انتشار : 1395
چکیده
Hyoscyamine and scopolamine are tropane alkaloids employed in medicine as anticholinergic agents and extracted from plants that belong to several species of Solanaceae because their chemical synthesis is complicated and expensive obtaining these compounds through cell suspension culture is a promising approach. The effect of Piriformospora indica, a plant growth-promoting root endophyte fungus, on tropane alkaloids production and expression of one rate-limiting enzyme gene, Putrescine N-methyltransferase (pmt) were studied in A. belladonna cell suspension culture. Based on our results the highest level of total alkaloids in MS + 1 mg.L-1 NAA (naphthalene acetic acid) + 1 mg.L-1 BA(6-Benzylaminopurin)…