Cloning and expression of human bone morphogenetic protein-2 gene in Leishmania tarentolae

Cloning and expression of human bone morphogenetic protein-2 gene in Leishmania tarentolae

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چکیدهSeveral expression hosts have been employed for the production of bone morphogenetic protein2 (BMP- 2), inducing de novo bone formation and is widely used in clinical applications. However,every expression system has its merits and demerits. For example, although Escherichia coli issimple and robust, but inept to make disulfide bond; hence, heterologous proteins are produced inthe form of inclusion bodies, entailing in vitro refolding strategies. On the other hand, yeast, whichis a lower eukaryote, is able to perform glycosylation of the recombinant proteins. However, itsglycosylation pattern is different from human beings. Ultimately, Leishmania tarentolae, a parasiteprotozoan of the lizard, unifying both…